Use of Enhanced Green Fluorescent Protein to Optimize and Quantitate Infection of Target Cells with Recombinant Retroviruses | Zendy

Linda Cashion | Zendy; Lance A. Bare | Zendy; Susan Harvey | Zendy; Quang Duy Trinh | Zendy; Yun Zhu | Zendy; James J. Devlin | Zendy

Open Access

Use of Enhanced Green Fluorescent Protein to Optimize and Quantitate Infection of Target Cells with Recombinant Retroviruses

Author(s) -

Linda Cashion,

Lance A. Bare,

Susan Harvey,

Quang Duy Trinh,

Yun Zhu,

James J. Devlin

Publication year - 1999

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/99265rr02

Subject(s) - recombinant dna , retrovirus , green fluorescent protein , flow cytometry , fluorescence , fluorescence microscope , biology , reporter gene , cell culture , titer , virology , microbiology and biotechnology , transfection , gene , gene expression , virus , biochemistry , genetics , physics , quantum mechanics

Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells. In this paper, we extended the use of the fluorescent reporter to quantify protein expression using such analytical tools as fluorescent microscopy, flow cytometry and fluorescent plate reader analysis. These tools enabled us to rapidly assess the titer of recombinant retrovirus harvested from packaging cells and to optimize parameters for infection of different cell lines.

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