Open AccessVersatile Epitope Tagging Vector for Gene Expression in Mammalian Cells
Author(s) -
Tanya Hosfield,
Quinn Lu
Publication year - 1998
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/98252pf01
Subject(s) - epitope , gene , vector (molecular biology) , biology , expression vector , gene expression , computational biology , gene product , microbiology and biotechnology , genetics , recombinant dna , antigen
We have constructed an epitope-tagging vector, pCMV-Tag1, for gene expression in mammalian cells. This vector, which allows for N-terminal, C-terminal and internal tagging of the gene product of interest with the FLAG and/or c-myc epitopes, enables researchers to rapidly and efficiently characterize gene products in vivo.
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