Open AccessQuick-FISH: A Rapid Fluorescence In Situ Hybridization Technique for Molecular Cytogenetic Analysis
Author(s) -
Sushanta K. Banerjee,
Allan P. Weston,
Diane L. Persons,
Donald R. Campbell
Publication year - 1998
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/98245dt03
Subject(s) - fluorescence in situ hybridization , fish <actinopterygii> , biology , in situ hybridization , in situ , molecular probe , microbiology and biotechnology , cytogenetics , hybridization probe , fluorescence , computational biology , genetics , dna , chemistry , chromosome , gene expression , gene , fishery , optics , physics , organic chemistry
A rapid and simplified fluorescence in situ hybridization (FISH) technique for the detection of human chromosome-specific centromeric probes is described. Using chromosomes 1-, 4-, 11- and Y-specific fluorescence-labeled probes, the modified, or quick-FISH technique, was compared to two conventional FISH methods. The modified FISH technique detected human chromosomes without sacrificing sensitivity or signal quality in both fresh-frozen and paraffin-embedded tissue sections. Further studies demonstrated that this technique can be used with simultaneous application of dual-color probes. This novel technique offers the advantages of being simpler to perform and faster than conventional techniques. The quick-FISH technique can be substituted for any sensitive conventional FISH method for molecular cytogenetic analysis in fresh, fresh-frozen or paraffin-embedded tissues.
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