Simultaneous Detection of Two GFP Spectral Mutants During In Vivo Confocal Microscopy of Migrating Dictyostelium Cells | Zendy

Timo Zimmermann | Zendy; Florian Siegert | Zendy

Open Access

Simultaneous Detection of Two GFP Spectral Mutants During In Vivo Confocal Microscopy of Migrating Dictyostelium Cells

Author(s) -

Timo Zimmermann,

Florian Siegert

Publication year - 1998

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/98243st06

Subject(s) - dictyostelium discoideum , green fluorescent protein , confocal microscopy , dictyostelium , confocal , fluorescence microscope , microscopy , microbiology and biotechnology , mutant , in vivo , biology , fluorescence , biophysics , chemistry , optics , biochemistry , gene , genetics , physics

A method is described that allows simultaneous measurement of two spectrally distinguishable green fluorescent protein (GFP) mutants with a confocal microscope. In contrast to previously described methods, neither UV excitation nor repetition of scans is required. Therefore the method is well-suited to the long-time observation of living cells in three-dimensional microscopy and time series recording, as demonstrated with GFP-expressing Dictyostelium discoideum cells.

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