ASD-GFP Vectors for In Vivo Expression Technology in Pseudomonas aeruginosa and Other Gram-Negative Bacteria | Zendy

Martin Handfield | Zendy; Herbert P. Schweizer | Zendy; Michael J. Mahan | Zendy; F. Sanschagrin | Zendy; Tung T. Hoang | Zendy; Roger C. Lévesque | Zendy

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ASD-GFP Vectors for In Vivo Expression Technology in Pseudomonas aeruginosa and Other Gram-Negative Bacteria

Author(s) -

Martin Handfield,

Herbert P. Schweizer,

Michael J. Mahan,

F. Sanschagrin,

Tung T. Hoang,

Roger C. Lévesque

Publication year - 1998

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/98242st02

Subject(s) - green fluorescent protein , biology , plasmid , bacteria , reporter gene , in vivo , pseudomonas aeruginosa , gene , gene expression , mutant , microbiology and biotechnology , selectable marker , genetics

We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate beta-semialdehyde dehydrogenase (asd) gene as a selectable marker and beta-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein with enhanced fluorescence properties (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria.

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