Preparation of PCR Products for DNA Sequencing | Zendy

Jack T. Leonard | Zendy; M.B. Grace | Zendy; Gregory S. Buzard | Zendy; Michael Mullen | Zendy; Cristina Barbagallo | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Preparation of PCR Products for DNA Sequencing

Author(s) -

Jack T. Leonard,

M.B. Grace,

Gregory S. Buzard,

Michael Mullen,

Cristina Barbagallo

Publication year - 1998

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/98242pf01

Subject(s) - polymerase chain reaction , dna , dna sequencing , biology , genetics , sequencing by ligation , computational biology , microbiology and biotechnology , base sequence , gene , genomic library

We demonstrate that routine PCR product analytical agarose gels can also serve as preparative gels for quick DNA template purification before sequencing. The band of interest is excised, placed into a Gel Nebulizer inside a Micropure separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research