Compact, Synthetic, Vaccinia Virus Early/Late Promoter for Protein Expression | Zendy

Sekhar Chakrabarti | Zendy; Jerry R. Sisler | Zendy; Bernard Moss | Zendy

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Compact, Synthetic, Vaccinia Virus Early/Late Promoter for Protein Expression

Author(s) -

Sekhar Chakrabarti,

Jerry R. Sisler,

Bernard Moss

Publication year - 1997

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/97236st07

Subject(s) - vaccinia , poxviridae , biology , virus , thymidine kinase , promoter , orthopoxvirus , virology , plasmid , gene , recombinant virus , vector (molecular biology) , expression vector , recombinant dna , gene expression , microbiology and biotechnology , genetics , herpes simplex virus

Vaccinia virus, a member of the poxvirus family, is widely used as a mammalian cell expression vector. Vaccinia virus replicates in the cytoplasm and has its own transcriptional system, making it necessary to use viral promoters. Here, we describe the design, construction and use of a 40-bp synthetic, vaccinia virus promoter with largely overlapping early and late regulatory elements. Convenient plasmid transfer vectors are depicted for expression of one or two genes under control of strong early/late promoters and allowing for thymidine kinase (TK) or antibiotic selection of recombinant viruses.

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