Direct PCR of Small Genomic DNA Fragments from Serum | Zendy

Andrew J. Sandford | Zendy; Peter D. Paré | Zendy

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Direct PCR of Small Genomic DNA Fragments from Serum

Author(s) -

Andrew J. Sandford,

Peter D. Paré

Publication year - 1997

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/97235st05

Subject(s) - genomic dna , genotyping , dna , microbiology and biotechnology , biology , polymerase chain reaction , multiple displacement amplification , dna extraction , gene , genetics , genotype

A simple and rapid method is described where human genomic DNA suitable for PCR was prepared from serum by microwave irradiation. We were able to reproducibly amplify single-copy gene sequences up to 442 bp from small quantities of serum without the need for DNA extraction. Genotyping results obtained from serum samples were shown to be identical to those derived from purified DNA from the same individuals.

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