Microplate Assay for Measurement of Histamine Release from Mast Cells | Zendy

Joseph A. Price | Zendy

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Microplate Assay for Measurement of Histamine Release from Mast Cells

Author(s) -

Joseph A. Price

Publication year - 1997

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/97225rr04

Subject(s) - histamine , reproducibility , mast (botany) , chemistry , chromatography , mast cell , microbiology and biotechnology , biology , immunology , pharmacology

A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.

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