Rainbow™ Universal CPG: A Versatile Solid Support for Oligonucleotide Synthesis | Zendy

Paul S. Nelson | Zendy; Sylvester Muthini | Zendy; Mike Vierra | Zendy; L. Acosta | Zendy; Thomas H. Smith | Zendy

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Rainbow™ Universal CPG: A Versatile Solid Support for Oligonucleotide Synthesis

Author(s) -

Paul S. Nelson,

Sylvester Muthini,

Mike Vierra,

L. Acosta,

Thomas H. Smith

Publication year - 1997

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/97224pf01

Subject(s) - oligonucleotide , cpg site , ammonium hydroxide , solid phase synthesis , combinatorial chemistry , oligonucleotide synthesis , nucleic acid , chemistry , dna , biochemistry , gene , organic chemistry , gene expression , peptide , dna methylation

We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3'-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.

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