Open AccessMethods and Applications of Three-Color Confocal Imaging
Author(s) -
Stephen W. Paddock,
Eric J. Hazen,
Peter J. DeVries
Publication year - 1997
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/97221st04
Subject(s) - confocal , confocal microscopy , computational biology , optics , biology , physics
A simple method for constructing two- and three-color merged images from grayscale confocal fluorescence images using Adobe Photoshop is outlined. Various computer methods for manipulating and displaying the images are discussed in light of several recent biomedical applications of multi-label confocal microscopy.
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