Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells | Zendy

Michael Eaton | Zendy; Jennifer E. Hardingham | Zendy; Dusan Kotasek | Zendy; Alexander Dobrovic | Zendy

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Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells

Author(s) -

Michael Eaton,

Jennifer E. Hardingham,

Dusan Kotasek,

Alexander Dobrovic

Publication year - 1997

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/97221st01

Subject(s) - biology , circulating tumor cell , polymerase chain reaction , tumor cells , microbiology and biotechnology , computational biology , gene , cancer research , genetics , cancer , metastasis

Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 10(6) leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment.

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