A Microbiological Assay for the Quantitative Determination of Glutathione | Zendy

Martin Schmidt | Zendy; Martin Grey | Zendy; Martin Brendel | Zendy

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A Microbiological Assay for the Quantitative Determination of Glutathione

Author(s) -

Martin Schmidt,

Martin Grey,

Martin Brendel

Publication year - 1996

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/96215st07

Subject(s) - glutathione , saccharomyces cerevisiae , quantitative analysis (chemistry) , biochemistry , strain (injury) , chemistry , enzyme , biology , chromatography , yeast , anatomy

Based on the requirement of a glutathione-deficient mutant strain of Saccharomyces cerevisiae to take up external glutathione for growth on synthetic media, a simple agar diffusion test for quantitative detection of total glutathione from various sources was established. Glutathione concentrations can be reliably detected in a less expensive way, requiring less technical effort compared to enzymatic or biochemical detection methods.

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