Protection of DNA During Preparative Agarose Gel Electrophoresis Against Damage Induced by Ultraviolet Light | Zendy

Dirk Gründemann | Zendy; Edgar Schömig | Zendy

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Protection of DNA During Preparative Agarose Gel Electrophoresis Against Damage Induced by Ultraviolet Light

Author(s) -

Dirk Gründemann,

Edgar Schömig

Publication year - 1996

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/96215rr02

Subject(s) - ethidium bromide , agarose , agarose gel electrophoresis , gel electrophoresis of nucleic acids , gel electrophoresis , dna , ultraviolet light , microbiology and biotechnology , chemistry , electrophoresis , guanosine , dna damage , biology , chromatography , biochemistry , photochemistry

Preparative gel electrophoresis of double-stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in vitro transcription, transformation of E. coli and PCR. Inhibition was not caused by enzyme-inhibiting impurities in the agarose gel, but was induced by a standard transilluminator fitted with 312-nm tubes. Interestingly, it was possible to protect the DNA against UV damage by the addition of cytidine or guanosine to the electrophoresis buffer.

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