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Preparative gel electrophoresis of double-stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in vitro transcription, transformation of E. coli and PCR. Inhibition was not caused by enzyme-inhibiting impurities in the agarose gel, but was induced by a standard transilluminator fitted with 312-nm tubes. Interestingly, it was possible to protect the DNA against UV damage by the addition of cytidine or guanosine to the electrophoresis buffer.

Protection of DNA During Preparative Agarose Gel Electrophoresis Against Damage Induced by Ultraviolet Light