Automated DNA Sequencing Requiring No DNA Template Purification | Zendy

Qianfa Chen | Zendy; Catherine Neville | Zendy; Alex MacKenzie | Zendy; Robert G. Korneluk | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Automated DNA Sequencing Requiring No DNA Template Purification

Author(s) -

Qianfa Chen,

Catherine Neville,

Alex MacKenzie,

Robert G. Korneluk

Publication year - 1996

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/96213st02

Subject(s) - sequencing by ligation , dna , dna nanoball sequencing , dna sequencing , biology , template , polymerase chain reaction , dna extraction , microbiology and biotechnology , computational biology , base sequence , genomic library , genetics , gene , nanotechnology , materials science

A critical component in automated fluorescent DNA sequencing is good quality of DNA template. In an effort to reduce the dependence of sequencing success on DNA template quality, the effect of heat-soaked PCR on automated sequencing reactions has been examined. We have found that the heat-soaked PCR protocol considerably improves the overall quality of sequence data and significantly reduces the dependence on the quality of DNA templates. The improvement is corroborated by our ability to obtain over 500 bp of readable sequence per reaction using DNA from E. coli lysates as template obviating DNA purification.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research