Efficiency of Transduction by Recombinant Sindbis Replicon Virus Varies Among Cell Lines, Including Mosquito Cells and Rat Sensory Neurons | Zendy

Joe Corsini | Zendy; Donald L. Traul | Zendy; C.L. Wilcox | Zendy; P. J. Gaines | Zendy; J. O. Carlson | Zendy

Open Access

Efficiency of Transduction by Recombinant Sindbis Replicon Virus Varies Among Cell Lines, Including Mosquito Cells and Rat Sensory Neurons

Author(s) -

Joe Corsini,

Donald L. Traul,

C.L. Wilcox,

P. J. Gaines,

J. O. Carlson

Publication year - 1996

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/96213rr02

Subject(s) - sindbis virus , biology , transduction (biophysics) , replicon , cell culture , heterologous , virology , alphavirus , virus , recombinant dna , microbiology and biotechnology , recombinant virus , transfection , gene , genetics , plasmid , rna , biochemistry

Recombinant alphaviruses have been used as vehicles for delivery and expression of heterologous genes in mammalian, avian and insect cell lines. We have used a Sindbis replicon virus (Sinreplac) able to express the E. coli lacZ gene to compare the efficiency of transduction in one insect, six mammalian cell lines and cultured rat dorsal neurons which apparently express beta-galactosidase over a 30-day time period. Results show that different cell lines were transduced with varying degrees of efficiency and that this efficiency could be improved in some cell lines by packaging the replicon with a helper derived from a more neurovirulent strain of Sindbis.

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