Heterogeneity of DNA Binding to Membranes Used in Quantitative Dot Blots

1.Liu, Z., D.B. Batt and G.G. Carmichael. 1994. Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA. Proc. Natl. Acad. Sci. USA 91:4258-4262. 2.Sawadogo, M. and R.G. Roeder. 1985. Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay. Proc. Natl. Acad. Sci. USA 82:4394-4398. 3.Taira, K., K. Nakagawa, S. Nishikawa and K. Furukawa. 1991. Construction of a novel RNA-transcript-trimming plasmid which can be used in vitro in place of run-off and (G)free transcriptions and in vivo as multi-sequences transcription vectors. Nucleic Acids Res. 19:5125-5130. 4.Yamazaki, K., F. Katagiri, H. Imaseki and N.H. Chua. 1990. TGA1a, a tobacco DNAbinding protein, increases the rate of preinitiation complex formation in a plant in vitro transcription system [corrected] [published erratum appears in Proc. Natl. Acad. Sci. USA 87:9508]. Proc. Natl. Acad. Sci. USA 87:7035-7039. This work was supported by the National Cancer Institute under Grant CA45382. The author would like to thank Xiotong Li for the construction of CMV-RZ. Address correspondence to David B. Batt, Pathology, Dana Farber Cancer Institute, Mayer 857, 44 Binney St., Boston, MA 02115, USA. Internet: david_batt@dfci.harvard. edu