Open AccessDirectional Enrichment of Directly Cloned PCR Products
Author(s) -
David Horn
Publication year - 2005
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/05391bm03
Subject(s) - polymerase chain reaction , cloning (programming) , biology , computational biology , molecular cloning , genetics , microbiology and biotechnology , gene , complementary dna , computer science , programming language
Reorienting Directional Cloning The cloning of PCR-amplified DNA fragments is frequently the first step in expression vector construction. Commercial kits that exploit the tendency of DNA polymeras...
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