Liquid Tissue™: Proteomic Profiling of Formalin-Fixed Tissues | Zendy

DaRue A. Prieto | Zendy; Brian L. Hood | Zendy; Marlene Darfler | Zendy; Thomas Guiel | Zendy; David A. Lucas | Zendy; Thomas P. Conrads | Zendy; Timothy D. Veenstra | Zendy; David B. Krizman | Zendy

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Liquid Tissue™: Proteomic Profiling of Formalin-Fixed Tissues

Author(s) -

DaRue A. Prieto,

Brian L. Hood,

Marlene Darfler,

Thomas Guiel,

David A. Lucas,

Thomas P. Conrads,

Timothy D. Veenstra,

David B. Krizman

Publication year - 2005

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/05386su06

Subject(s) - computational biology , mass spectrometry , proteomics , biomarker , proteome , biomarker discovery , profiling (computer programming) , biology , chromatography , chemistry , bioinformatics , computer science , biochemistry , gene , operating system

Identification and quantitation of candidate biomarker proteins in large numbers of individual tissues is required to validate specific proteins, or panels of proteins, for clinical use as diagnostic, prognostic, toxicological, or therapeutic markers. Mass spectrometry (MS) provides an exciting analytical methodology for this purpose. Liquid Tissue MS protein preparation allows researchers to utilize the vast, already existing, collections offormalin-fixed paraffin-embedded (FFPE) tissues for the procurement of peptides and the analysis across a variety of MS platforms.

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