Brief Heat Shock Increases Stable Integration of Lipid-Mediated DNA Transfections | Zendy

Brian L. Pipes | Zendy; Farha H. Vasanwala | Zendy; Tom C. Tsang | Zendy; Tong Zhang | Zendy; Phoebe Luo | Zendy; David T. Harris | Zendy

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Brief Heat Shock Increases Stable Integration of Lipid-Mediated DNA Transfections

Author(s) -

Brian L. Pipes,

Farha H. Vasanwala,

Tom C. Tsang,

Tong Zhang,

Phoebe Luo,

David T. Harris

Publication year - 2005

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/05381bm05

Subject(s) - transfection , plasmid , dna , gene transfer , gene , library science , biology , genetics , computer science

1.Lin Cereghino, J. and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:45-66. 2.Cregg, J.M., K.J. Barringer, A.Y. Hessler, and K.R. Madden. 1985. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5:3376-3385. 3.Cregg, J.M. and K.A. Russell. 1998. Transformation methods, p. 27-39. In D.R. Higgins and J.M. Cregg (Eds.), Methods in Molecular Methods Molecular Biology, Vol. 103: Pichia Protocols. Humana Press, Totowa, NJ. 4.Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168. 5.Dohmen, R.J., A.W.M. Strasser, C.B. Honer, and C.P. Hollenberg. 1991. An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 7:691-692.

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