DNA Nucleic Acid Sequence-Based Amplification-Based Genotyping for Polymorphism Analysis | Zendy

Cecile Berard | Zendy; Marie-Angélique Cazalis | Zendy; Philippe Leissner | Zendy; Bruno Mougin | Zendy

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DNA Nucleic Acid Sequence-Based Amplification-Based Genotyping for Polymorphism Analysis

Author(s) -

Cecile Berard,

Marie-Angélique Cazalis,

Philippe Leissner,

Bruno Mougin

Publication year - 2004

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/04374dd04

Subject(s) - nasba , genotyping , molecular inversion probe , biology , molecular beacon , multiplex , dna , snp genotyping , dna nanoball sequencing , computational biology , nucleic acid , locked nucleic acid , dna microarray , genomic dna , microbiology and biotechnology , genetics , nucleic acid sequence , genomic library , genotype , oligonucleotide , gene , base sequence , gene expression

Nucleic acid sequence-based amplification (NASBA) is a sensitive isothermal transcription-based amplification method known to be a suitable tool for RNA research. We demonstrate that NASBA technology can be applied to single nucleotide polymorphism (SNP) analysis using human genomic DNA as a template. Combination of DNA NASBA with multiplex hybridization of specific molecular beacons makes it possible to unambiguously discriminate the presence of the SNP of interest. This protocol is easy-to-use, robust, and makes it possible to rapidly detect single nucleotide substitutions in clinical or cell line DNA sequences using a large range of DNA input. Such a real-time genotyping DNA NASBA assay can find broad application in clinical diagnostics.

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