Open AccessProcedure for controlling number of repeats, orientation, and order during cloning of oligonucleotides
Author(s) -
Eran Blachinsky,
Irit Marbach,
Ruth Cohen,
Melanie R. Grably,
David Engelberg
Publication year - 2004
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/04366bm02
Subject(s) - oligonucleotide , cloning (programming) , biology , computational biology , genetics , orientation (vector space) , dna , molecular cloning , microbiology and biotechnology , computer science , mathematics , gene , complementary dna , geometry , programming language
COOL Cloning Insertion of short DNA sequences into plasmids is a widely applied technique, but it can be complicated by lack of precision and the need to screen and separate clones in order to iden...
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