Isolation of high-quality RNA from gymnosperm and angiosperm trees | Zendy

Natalia Kolosova | Zendy; Barbara Miller | Zendy; Steven Ralph | Zendy; Brian E. Ellis | Zendy; Carl J. Douglas | Zendy; Kermit Ritland | Zendy; Jörg Bohlmann | Zendy

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Isolation of high-quality RNA from gymnosperm and angiosperm trees

Author(s) -

Natalia Kolosova,

Barbara Miller,

Steven Ralph,

Brian E. Ellis,

Carl J. Douglas,

Kermit Ritland,

Jörg Bohlmann

Publication year - 2004

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/04365st06

Subject(s) - gymnosperm , isolation (microbiology) , biology , rna , gene , botany , genetics , microbiology and biotechnology

An improved protocol was developed for efficient and reliable extraction of high-quality total RNA and mRNA from various tissues of spruce (Picea spp.) and poplar (Populus spp.) trees, as well as other plant species. This method was specifically optimized for tissues with high content of polysaccharides, oleoresin terpenoids, and phenolic secondary metabolites, which often co-precipitate with RNA and inhibit subsequent reverse transcription. The improved protocol yielded up to 600 micrograms of total RNA per gram of tissue suitable for standard expressed sequence tags (ESTs), full-length cDNA library construction, and for microarray applications.

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