Production and assay of a transcription activator CRP in coupled in vitro transcription and translation

BENCHMARKS 9q and 20, where, respectively, chromo-somal loss and gain is frequently detected in SCA III ovarian tumors (7,8). After selecting the genes on these respective chromosomes, hierarchical clustering is performed. Sorting the genes on chromosomal order reveals particular chromosomal regions with under-or overexpressed genes in subsets of SCA III tumors. Possible retention or amplification on chromosome 9p in all tumors and predominant chromosomal loss on chromosome 9q in a number of SCA III tumors is observed. When analyzed in the same way, chromosome 20 reveals potential amplified regions in the majority of tumors (Figure 1, B and C). Application of this method gives direct insight into the chromosomal distribution of up-or down-regulated genes. It reveals continuous regions with gene expression biases. The visu-alization of these biases is a first step in better understanding of the relations between physical loss or gain of chromosomal regions and the effects of gene expression of genes located in these regions. This observation may also affect the significance of micro-array expression data. Up-regulating of a gene within a region of other up-regulated genes may be due to genomic instability and can be less significant than a solitary up-regulated gene. The macro and the user manual are available from the authors at