Indirect measurements of differential gene expression with cDNA microarrays | Zendy

Thomas J. Belbin | Zendy; John M. Gaspar | Zendy; Missak Haigentz | Zendy; Román Pérez-Soler | Zendy; Steven M. Keller | Zendy; Michael B. Prystowsky | Zendy; Geoffrey Childs | Zendy; Nicholas D. Socci | Zendy

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Indirect measurements of differential gene expression with cDNA microarrays

Author(s) -

Thomas J. Belbin,

John M. Gaspar,

Missak Haigentz,

Román Pérez-Soler,

Steven M. Keller,

Michael B. Prystowsky,

Geoffrey Childs,

Nicholas D. Socci

Publication year - 2004

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/04362mt02

Subject(s) - complementary dna , dna microarray , false positive paradox , biology , gene expression , gene , rna , genetics , microarray , computational biology , reference genes , microbiology and biotechnology , gene expression profiling , mathematics , statistics

The use of universal RNA reference sets is an increasingly common approach to molecular classification studies with cDNA microarrays. Here we evaluated the reliability of indirect measurements of fluorescence ratios with a common RNA reference as a means of identifying differentially expressed genes. Comparisons of direct and indirect measures of differential gene expression showed a strong overall correlation in fluorescence ratio measurements but also a high degree of false positives in our indirect measurements. These results indicated that the application of more stringent ratio filters may be required when assessing differential gene expression utilizing a common RNA reference in classification studies.

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