Open AccessSodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis
Author(s) -
Jonathan R. Brody,
Scott E. Kern
Publication year - 2004
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/04362bm02
Subject(s) - boric acid , tris , gel electrophoresis , dna , sodium , electrophoresis , chemistry , chromatography , biochemistry , organic chemistry
age (5−10 V/cm) runs necessary and highlights the limitations of using Tris as a cation for DNA electrophoresis. Another component of currently used buffers, EDTA, is now largely superfluous, since most DNA samples are readily soluble and since commonly used enzymes today would not carry an undesirable enzymatic activity under electrophoretic conditions.
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