Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome | Zendy

Dong P. Liu | Zendy; Cecilia Schmidt | Zendy; Timothy Billings | Zendy; Muriel T. Davisson | Zendy

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Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome

Author(s) -

Dong P. Liu,

Cecilia Schmidt,

Timothy Billings,

Muriel T. Davisson

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03356st02

Subject(s) - genotyping , biology , microbiology and biotechnology , real time polymerase chain reaction , phenotype , fluorescence in situ hybridization , fish <actinopterygii> , genetics , in situ hybridization , genotype , gene , messenger rna , chromosome , fishery

The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genotyped.

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