High-throughput isolation of recombinant antibodies against recombinant allergens | Zendy

Claudio Rhyner | Zendy; Zoltán Konthur | Zendy; Kurt Blaser | Zendy; Reto Crameri | Zendy

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High-throughput isolation of recombinant antibodies against recombinant allergens

Author(s) -

Claudio Rhyner,

Zoltán Konthur,

Kurt Blaser,

Reto Crameri

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03354bm01

Subject(s) - phage display , recombinant dna , complementary dna , biology , antibody , cdna library , microbiology and biotechnology , computational biology , virology , gene , genetics

of recombinant allergens and the need for sensitive reagents for their detection prompted us to implement high-throughput technologies (10) for the rapid selection of allergen-specific antibodies. Starting from “single pot” naive human antibody libraries that have been previously described (11), we performed parallel selections of phage carrying functional, binding single-chain fragment variables (scFvs) against different

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