Analysis of histone deposition on specific DNA elements in living mammalian cells | Zendy

Laetitia Daury | Zendy; Didier Trouche | Zendy

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Analysis of histone deposition on specific DNA elements in living mammalian cells

Author(s) -

Laetitia Daury,

Didier Trouche

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03352st05

Subject(s) - histone , chromatin , histone modifying enzymes , biology , histone code , microbiology and biotechnology , chromatin immunoprecipitation , histone h2a , histone h1 , dna , dna replication , genetics , nucleosome , gene expression , gene , promoter

DNA replication and repair involve the deposition of newly synthesized histones. After their deposition on a specific locus, histones are extensively modified on their protruding N-terminal tails to restore the histone code corresponding to this locus. These processes require a complex machinery of histone chaperones and histone-modifying enzymes. The precise kinetics of these events and the identity of the factors involved are still not clear. Here we present a technique based on transient expression of tagged histones followed by chromatin immunoprecipitation that allows the detection of newly synthesized histone deposition on specific DNA elements in mammalian cells at high resolution. This strategy could have important applications in chromatin dynamic studies.

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