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AATCC-3′. When double-stranded, these oligonucleotides have termini that are cohesive with the ends of HindIII-linearized pFOS-1. However, the HindIII site is not regenerated upon insertion of the oligonucleotide duplex into the vector. The central region of the duplex contains recognition sites for two restriction enzymes that leave blunt ends, SwaI and PmlI. The modified vector pFOS-LA was

pFOS-LA: a modified vector for production of random shear fosmid libraries