Open AccesspFOS-LA: a modified vector for production of random shear fosmid libraries
Author(s) -
Jonathan L. Longmire,
Nancy C. Brown
Publication year - 2003
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/03351bm04
Subject(s) - national laboratory , physics , fosmid , library science , computer science , engineering physics , biology , genetics , genome , gene
AATCC-3′. When double-stranded, these oligonucleotides have termini that are cohesive with the ends of HindIII-linearized pFOS-1. However, the HindIII site is not regenerated upon insertion of the oligonucleotide duplex into the vector. The central region of the duplex contains recognition sites for two restriction enzymes that leave blunt ends, SwaI and PmlI. The modified vector pFOS-LA was
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