Microarray-Based Method for Combinatorial Library Sequence Mapping and Characterization | Zendy

Valérie Abécassis | Zendy; Loïc Jaffrelo | Zendy; David S. Rickman | Zendy; Lawrence P. Aggerbeck | Zendy; C.J. Herbert | Zendy; Gilles Truan | Zendy; Denis Pompon | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Microarray-Based Method for Combinatorial Library Sequence Mapping and Characterization

Author(s) -

Valérie Abécassis,

Loïc Jaffrelo,

David S. Rickman,

Lawrence P. Aggerbeck,

C.J. Herbert,

Gilles Truan,

Denis Pompon

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03346mt03

Subject(s) - oligonucleotide , dna microarray , oligomer restriction , sequence (biology) , computational biology , biology , shuffling , dna , dna sequencing , sequence analysis , genetics , microbiology and biotechnology , gene , computer science , gene expression , programming language

Here we describe a DNA-chip-based method for high-throughput sequence mapping. This involves competitive hybridization between short and differentially labeled fluorescent oligonucleotide probes and glass-supported PCR products. Competition between an excess of oligonucleotide probes targeting the same sequence segment improves sequence discrimination and reduces sensitivity to experimental conditions such as probe concentrations, hybridization, and washing temperatures and durations. The method was found to be particularly adapted to sequence mapping of combinatorial libraries obtained by DNA shuffling between members of a gene family. We present an application of this technique for the characterization of recombination biases in combinatorial libraries used in directed evolution.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research