Kindred DNA Amplification from Two Distinct Populations of cDNA Fragments | Zendy

Ülo Puurand | Zendy; Lumme Kadaja | Zendy; Enn Seppet | Zendy

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Kindred DNA Amplification from Two Distinct Populations of cDNA Fragments

Author(s) -

Ülo Puurand,

Lumme Kadaja,

Enn Seppet

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03345st06

Subject(s) - suppression subtractive hybridization , complementary dna , biology , southern blot , microbiology and biotechnology , dna , genetics , molecular cloning , rapid amplification of cdna ends , cloning (programming) , gene duplication , polymerase chain reaction , dna–dna hybridization , nucleic acid thermodynamics , hybridization probe , gene , cdna library , base sequence , computer science , programming language

Kindred DNA amplification is a novel and cost-effective method developed to isolate common cDNA fragments between two distinct cDNA populations. Unlike subtractive hybridization, which discards common sequences, kindred DNA amplification isolates and amplifies these sequences within a single hybridization procedure. The utility of this method is demonstrated by cloning the genes in common between two different but metabolically homologous muscles, murine ventricular myocardium and soleus. The reliability of kindred DNA amplification was confirmed by Southern hybridization.

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