Chemical RNA Labeling without 3′ End Bias Using Fluorescent cis -Platin Compounds | Zendy

R.S. Hagedoorn | Zendy; Richard W. Joseph | Zendy; Soemini Kasanmoentalib | Zendy; PHC Eilers | Zendy; Jeffrey B. Killian | Zendy; Anton K. Raap | Zendy

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Chemical RNA Labeling without 3′ End Bias Using Fluorescent cis -Platin Compounds

Author(s) -

R.S. Hagedoorn,

Richard W. Joseph,

Soemini Kasanmoentalib,

PHC Eilers,

Jeffrey B. Killian,

Anton K. Raap

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03345st03

Subject(s) - oligonucleotide , nucleic acid , fluorescence , microbiology and biotechnology , rna , dna microarray , fluorescent labelling , biology , reverse transcriptase , transcription (linguistics) , chemistry , dna , biochemistry , gene expression , gene , physics , linguistics , philosophy , quantum mechanics

Recently, fluorescent, monofunctional cis-platin derivatives have been developed to chemically label nucleic acids for use in fluorescent hybridization assays. Here we show by hybridizations to microarrays containing oligonucleotide probes for the 3' ends, middle parts, and 5' ends of mRNAs, that this labeling methodology bypasses the problem of the 3' end bias that is characteristic of the conventional enzymatic oligo(dT)-primed, reverse transcription labeling of mRNAs.

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