Single-Stranded Conformational Polymorphism Analysis Using Automated Capillary Array Electrophoresis Apparatuses | Zendy

Shingo Baba | Zendy; Yoji Kukita | Zendy; Koichiro Higasa | Zendy; Tomoko Tahira | Zendy; Kenshi Hayashi | Zendy

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Single-Stranded Conformational Polymorphism Analysis Using Automated Capillary Array Electrophoresis Apparatuses

Author(s) -

Shingo Baba,

Yoji Kukita,

Koichiro Higasa,

Tomoko Tahira,

Kenshi Hayashi

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03344st03

Subject(s) - single strand conformation polymorphism , capillary electrophoresis , dna sequencer , single nucleotide polymorphism , allele , biology , str analysis , genetics , polymorphism (computer science) , computational biology , dna , microbiology and biotechnology , microsatellite , polymerase chain reaction , genotype , dna sequencing , gene

We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI Prism 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations and/or polymorphisms in different capillaries or different runs can be reliably detected. The relative peak heights between alleles are quantitative and reproducible between runs, and so allele frequencies of single nucleotide polymorphisms can be accurately estimated by a pooled DNA strategy. The method allows unattended, low-cost, and quantitative SSCP analysis using instruments that are widely accessible.

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