Reverse-Polarity PAGE for Examining DNA Binding Domain Phosphorylation | Zendy

Christopher D. Deppmann | Zendy; Elizabeth J. Taparowsky | Zendy

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Reverse-Polarity PAGE for Examining DNA Binding Domain Phosphorylation

Author(s) -

Christopher D. Deppmann,

Elizabeth J. Taparowsky

Publication year - 2003

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/03341bm08

Subject(s) - library science , social media , domain (mathematical analysis) , computer science , world wide web , mathematics , mathematical analysis

1.Barik, S. and M.S. Galinski. 1991. “Megaprimer” method of PCR: increased template concentration improves yield. BioTechniques 10:489-490. 2.Colosimo, A., Z. Xu, G. Novelli, B. Dallapiccola, and D.C. Gruenert. 1999. Simple version of “megaprimer” PCR for site-directed mutagenesis. BioTechniques 26:870-873. 3.Datta, A.K. 1995. Efficient amplification using “megaprimer” by asymmetric polymerase chain reaction. Nucleic Acids Res. 23:45304531. 4.Giebel, L.B. and R.A. Spritz. 1990. Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer. Nucleic Acids Res. 25:4947. 5.Ling, M.M. and B.H. Robinson. 1997. Approaches to DNA mutagenesis: an overview. Anal. Biochem. 254:157-178. 6.Sarkar, G. and S.S. Sommer. 1990. The “megaprimer” method of site-directed mutagenesis. BioTechniques 8:404-407. 7.Smith, A.M. and K.P. Klugman. “Megaprimer” method of PCR-based mutagenesis: the concentration of megaprimer is a critical factor. BioTechniques 22:438-442.

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