Ink-Jet Printer Heads for Ultra-Small-Drop Protein Crystallography | Zendy

Eduardo Howard | Zendy; Raúl E. Cachau | Zendy

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Ink-Jet Printer Heads for Ultra-Small-Drop Protein Crystallography

Author(s) -

Eduardo Howard,

Raúl E. Cachau

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02336pt03

Subject(s) - drop (telecommunication) , volume (thermodynamics) , protein crystallization , materials science , 3d printer , crystallization , nanotechnology , crystallography , computer science , chemistry , physics , mechanical engineering , engineering , telecommunications , organic chemistry , quantum mechanics

Mass-produced automated piezoelectric driven picoliter delivery systems (printer heads) are fast, inexpensive, and reliable devices that are capable of delivering a very large range of volumes and are ideally suited for high-throughput protein crystallography studies. We used this technology to set up under-oil crystallization experiments with drop sizes from the 200-nL to 3-microL volume range, commonly used in protein crystallography, and show its application in setting ultra-small (2 nL) drops, the smallest drop volume reported to date for this type of assay.

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