Express Primer Tool for High-Throughput Gene Cloning and Expression | Zendy

Jennifer Yoon | Zendy; Philip D. Laible | Zendy; M. Gu | Zendy; Heather N. Scott | Zendy; F. Collart | Zendy

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Express Primer Tool for High-Throughput Gene Cloning and Expression

Author(s) -

Jennifer Yoon,

Philip D. Laible,

M. Gu,

Heather N. Scott,

F. Collart

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02336bc03

Subject(s) - primer (cosmetics) , cloning (programming) , throughput , computational biology , biology , gene , flexibility (engineering) , genetics , computer science , programming language , operating system , chemistry , statistics , mathematics , organic chemistry , wireless

High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.

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