Negative Purification Method for the Selection of Specific Antibodies from Polyclonal Antisera | Zendy

Cindy Benet | Zendy; Pierre Van Cutsem | Zendy

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Negative Purification Method for the Selection of Specific Antibodies from Polyclonal Antisera

Author(s) -

Cindy Benet,

Pierre Van Cutsem

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02335st06

Subject(s) - polyclonal antibodies , antiserum , antibody , selection (genetic algorithm) , specific antibody , chemistry , biology , microbiology and biotechnology , immunology , computer science , artificial intelligence

We developed a protocol to remove nonspecific antibodies from polyclonal antisera by adsorption on non-target antigens immobilized on nitrocellulose membranes. This “negative” purification method is simple and provides better immunoreagents than the blocking of nonspecific antibodies in solution or the enrichment of specific antibodies on nitrocellulose membranes. For routine applications, this method is quicker and cheaper than the purification protocols based on selective precipitations and affinity chromatography.

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