Broad-Host-Range Mobilizable Suicide Vectors for Promoter Trapping in Gram-Negative Bacteria | Zendy

Mounir Izallalen | Zendy; Roger C. Lévesque | Zendy; Xavier Perret | Zendy; W. J. Broughton | Zendy; Hani Antoun | Zendy

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Broad-Host-Range Mobilizable Suicide Vectors for Promoter Trapping in Gram-Negative Bacteria

Author(s) -

Mounir Izallalen,

Roger C. Lévesque,

Xavier Perret,

W. J. Broughton,

Hani Antoun

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02335st04

Subject(s) - kanamycin , plasmid , tetracycline , biology , gene , green fluorescent protein , bacteria , vector (molecular biology) , gentamicin , genetics , selectable marker , microbiology and biotechnology , antibiotics , recombinant dna

Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to follow the induction of gene expression by measuring chloramphenicol resistance and fluorescence, respectively.

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