In Vivo Functional Proteomics: Mammalian Genome Annotation Using CD-Tagging | Zendy

Jonathan W. Jarvik | Zendy; Gregory W. Fisher | Zendy; C. Shi | Zendy; L. Hennen | Zendy; Christopher R. Hauser | Zendy; Sally A. Adler | Zendy; Peter B. Berget | Zendy

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In Vivo Functional Proteomics: Mammalian Genome Annotation Using CD-Tagging

Author(s) -

Jonathan W. Jarvik,

Gregory W. Fisher,

C. Shi,

L. Hennen,

Christopher R. Hauser,

Sally A. Adler,

Peter B. Berget

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02334rr02

Subject(s) - proteome , biology , genome , proteomics , computational biology , green fluorescent protein , gene , genome project , epitope , genetics , microbiology and biotechnology , antigen

A self-inactivating CD-tagging retroviral vector was used to introduce epitope and GFP tags into genes and proteins in NIH 3T3 cells. Several hundred cell clones, each expressing GFP fluorescence in a distinctive pattern, were isolated. Molecular analysis showed that a wide variety of genes and proteins, some known and some newly discovered, had been tagged. The analysis also revealed that, in the great majority of instances, the abundance and cellular location of the tagged protein mirrored that of its untagged counterpart. This approach provides a systematic means for the functional annotation of mammalian genomes and proteomes in living cells.

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