Robust and Sensitive Nylon Hybridization Membrane Suitable for High-Throughput Robotic Arraying Applications | Zendy

M.T. Green | Zendy; N.C. Maller | Zendy; Steven Richmond | Zendy; D. Morris | Zendy; P.R. Ball | Zendy; Sarah K. Stephens | Zendy

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Robust and Sensitive Nylon Hybridization Membrane Suitable for High-Throughput Robotic Arraying Applications

Author(s) -

M.T. Green,

N.C. Maller,

Steven Richmond,

D. Morris,

P.R. Ball,

Sarah K. Stephens

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02332ht04

Subject(s) - membrane , stripping (fiber) , sensitivity (control systems) , dna–dna hybridization , chromatography , dna , materials science , biology , chemistry , biochemistry , engineering , electronic engineering , composite material

An important aspect of automated macroarraying is the suitability of the nylon membrane selected on which samples are to be arrayed. PerForma is a positively charged nylon membrane that has been developed specificallyfor automated macroarraying. Tests usingfluorescent hybridization detection methods have shown that immobilized DNA amounts as low as 0.25 pg can be detected and that positive signals are obtainable after 21 stripping cycles. This report describes the improved colony growth, improved handling characteristics, increased hybridization detection sensitivity, and increased stripping and reprobing capability obtained using PerForma.

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