Paradoxical Effect of Eukaryotic Expression Vectors on Reporters | Zendy

Qiyue Hu | Zendy; Kiyofumi Suzuki | Zendy; Iwona HirschlerLaszkiewicz | Zendy; Lawrence I. Rothblum | Zendy

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Paradoxical Effect of Eukaryotic Expression Vectors on Reporters

Author(s) -

Qiyue Hu,

Kiyofumi Suzuki,

Iwona HirschlerLaszkiewicz,

Lawrence I. Rothblum

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02331st04

Subject(s) - transfection , vector (molecular biology) , reporter gene , expression vector , biology , microbiology and biotechnology , vector control , computational biology , genetics , gene expression , cell culture , gene , physics , recombinant dna , quantum mechanics , voltage , induction motor

A critical issue in transfection or cotransfection experiments is to define the appropriate controls. In most cases, a corresponding empty vector is used as one control. We report a paradoxical effect of empty mammalian expression vectors on different reporters. We have found that different empty vectors can inhibit or stimulate the same reporter. In addition, the same vector can have different effects on different reporters. This situation is further complicated by the observation that the effects of a vector on a reporter can vary depending on the cells used.

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