Storage of Various Cell Lines at -70°C or -80°C in Multi-Well Plates While Attached to the Substratum

layer to support the sample, we frequently measure proton currents traveling towards the dish floor, suggesting that this provides a more physiological environment in which ions are free to move from all surfaces of the ovariole unimpeded (Figure 3). The recording chamber provides a physiological environment and specimen manipulation and orientation possibilities that are well suited to extracellular electrophysiological investigation of a variety of tissues and can be adapted to other studies requiring precise sample orientation.