High-Throughput Array Production Using Precision Glass Syringes | Zendy

Jean Shieh | Zendy; C. Y. To | Zendy; J. Carramao | Zendy; Norihiro Nishimura | Zendy; Y. Maruta | Zendy; Y. Hashimoto | Zendy; David J. Wright | Zendy; Hsin-Hui Wu | Zendy; Arezou Azarani | Zendy

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High-Throughput Array Production Using Precision Glass Syringes

Author(s) -

Jean Shieh,

C. Y. To,

J. Carramao,

Norihiro Nishimura,

Y. Maruta,

Y. Hashimoto,

David J. Wright,

Hsin-Hui Wu,

Arezou Azarani

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02326mt07

Subject(s) - membrane , spots , spotting , membrane filter , materials science , chromatography , optics , chemistry , physics , biochemistry

The advantages of using 1, 96, or 384 precision glass syringes in automated high-throughput microdispensers in creating highly uniform and reproducible DNA, protein, and organic compound array filters and slides are described. Using the Hydra Microdispenser and Tango Liquid Handling system, 0.1-5 ng (in 50-300 nL) PCR-amplified, human cancer-related genes and housekeeping genes were spotted onto nylon membranes and coated slides. Protein solutions of 50 microg/mL to 1 mg/mL were spotted onto coated slides or onto MaxiSorp 96-well plates. Up to 6144 spots/membrane and up to 1000 spots/slide were printed. The size of the spots created by glass syringes was uniform and reproducible (precision variation of less than 5%) from spot to spot and membrane to membrane. Using a Tango 384 system, a total of ten 6144-spot filters can be produced in approximately 25 min, translating into a spotting speed of 2.5 min/membrane.

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