Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium by E. coli Expressing a Metallothionein Fused to β-Galactosidase | Zendy

Naoto Yoshida | Zendy; Tomohisa Kato | Zendy; Takashi Yoshida | Zendy; Kazunori Ogawa | Zendy; Mitsuo Yamashita | Zendy; Y Murooka | Zendy

Open Access

Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium by E. coli Expressing a Metallothionein Fused to β-Galactosidase

Author(s) -

Naoto Yoshida,

Tomohisa Kato,

Takashi Yoshida,

Kazunori Ogawa,

Mitsuo Yamashita,

Y Murooka

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02323st08

Subject(s) - metallothionein , recombinant dna , cadmium , fusion protein , cytoplasm , escherichia coli , cell culture , microbiology and biotechnology , chemistry , biology , biochemistry , gene , genetics , organic chemistry

We investigated the potential utility of a recombinant E. coli that expresses the human metallothionein II gene as a fusion protein with beta-galactosidase as a heavy metal biosorbent. E. coli cells expressing the metallothionein fusion demonstrated enhanced binding of Cd2+ compared to cells that lack the metallothionein. It was shown that the metallothionein fusion was capable of efficiently removing Cd2+ from solutions. Approximately 40% of the Cd2+ accumulated by the recombinant cells free in suspension was associated with the outer cell membrane, and 60% of that was present in the cytoplasm.

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