Epitope Tagging Genomic DNA Using a CD-Tagging Tn10 Minitransposon | Zendy

Cheryl A. Telmer | Zendy; Peter B. Berget | Zendy; Byron Ballou | Zendy; Robert F. Murphy | Zendy; Jonathan W. Jarvik | Zendy

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Epitope Tagging Genomic DNA Using a CD-Tagging Tn10 Minitransposon

Author(s) -

Cheryl A. Telmer,

Peter B. Berget,

Byron Ballou,

Robert F. Murphy,

Jonathan W. Jarvik

Publication year - 2002

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/02322rr04

Subject(s) - biology , transposable element , gene , nucleolin , genomic dna , epitope , tn10 , genetics , transfection , microbiology and biotechnology , dna , computational biology , genome , antigen , cytoplasm , nucleolus

Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a λFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

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