DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications | Zendy

Vladimir V. Didenko | Zendy

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DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications

Author(s) -

Vladimir V. Didenko

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01315rv02

Subject(s) - förster resonance energy transfer , oligonucleotide , dna , fluorescence , energy transfer , chromophore , biophysics , chemistry , computational biology , nanotechnology , biology , materials science , physics , biochemistry , photochemistry , optics , chemical physics

Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET. The approaches used in the design of new DNA FRET probes are discussed.

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