Microarray RNA Quantification Assay for Large-Scale Nanosamples Analysis | Zendy

Ilaria Duprè | Zendy; Michèle El Atifi | Zendy; Béatrice Rostaing | Zendy; E.M. Chambaz | Zendy; A.L. Benabid | Zendy; François Berger | Zendy

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Microarray RNA Quantification Assay for Large-Scale Nanosamples Analysis

Author(s) -

Ilaria Duprè,

Michèle El Atifi,

Béatrice Rostaing,

E.M. Chambaz,

A.L. Benabid,

François Berger

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01314mt03

Subject(s) - rna , reproducibility , microbiology and biotechnology , dna microarray , dna , biology , gene expression , computational biology , microarray , spotting , hybridization probe , rna extraction , chromatography , chemistry , gene , genetics , computer science , artificial intelligence

We have developed a convenient and sensitive method for the quantification of RNA in samples from microbiopsies. This procedure is especially suitable for quantitating very small amounts of RNA in largescale biological samples. This method, using a microarray-spotting facility for the study of multigenic expression, entails the hybridization of a DNA probe with RNA spotted at high density on nylon membrane. Furthermore, with this procedure, the reproducibility, sensitivity, and accuracy of the assay are notably improved as compared to current methods.

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