Sequence Verification as Quality-Control Step for Production of cDNA Microarrays | Zendy

Ellen Taylor | Zendy; David Cogdell | Zendy; Kevin R. Coombes | Zendy; Limei Hu | Zendy; Latha Ramdas | Zendy; A Tabor | Zendy; Stanley R. Hamilton | Zendy; W. Zhang | Zendy

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Sequence Verification as Quality-Control Step for Production of cDNA Microarrays

Author(s) -

Ellen Taylor,

David Cogdell,

Kevin R. Coombes,

Limei Hu,

Latha Ramdas,

A Tabor,

Stanley R. Hamilton,

W. Zhang

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01311st01

Subject(s) - genbank , complementary dna , sequence (biology) , sequence database , clone (java method) , expressed sequence tag , biology , dna microarray , cdna library , sequence analysis , computational biology , genetics , contig , microarray , gene , gene expression , genome

To generate cDNA arrays in our core laboratory, we amplified about 2300 PCR products from a human, sequence-verified cDNA clone library. As a quality-control step, we sequenced the PCR products immediately before printing. The sequence information was used to search the GenBank ® database to confirm the identities. Although these clones were previously sequence verified by the company, we found that only 79% of the clones matched the original database after handling. Our experience strongly indicates the necessity to sequence verify the clones at the final stage before printing on microarray slides and to modify the gene list accordingly.

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