Development of Ligase-Assisted Spacer Addition for the Measurement of Microsatellites | Zendy

Veronica Brockhurst | Zendy; Ross Barnard | Zendy; L. Wolter | Zendy; Philip M. Giffard | Zendy; Peter Timms | Zendy

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Development of Ligase-Assisted Spacer Addition for the Measurement of Microsatellites

Author(s) -

Veronica Brockhurst,

Ross Barnard,

L. Wolter,

Philip M. Giffard,

Peter Timms

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01311rr01

Subject(s) - microsatellite , genotyping , dna ligase , biology , computational biology , polyacrylamide gel electrophoresis , fractionation , dna , microbiology and biotechnology , genetics , chromatography , chemistry , genotype , biochemistry , enzyme , gene , allele

Conventional methods for detecting differences in microsatellite repeat lengths rely on electrophoretic fractionation on long denaturing polyacrylamide gels, a time-consuming and labor-intensive method. Therefore, there is a need for the development of new and rapid approaches to routinely detect such length polymorphisms. The advent of techniques allowing the coupling of DNA molecules to solid surfaces has provided new prospects in the area of mutation detection. We describe here the development and optimization of the ligase-assisted spacer addition (LASA) method, a novel and rapid procedure based on an ELISA format to measure microsatellite repeat lengths. The LASA assay was successfully applied to a set of 11 bird samples to assess its capabilities as a genotyping method.

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